Protocol SP1 --- Page 4 of 6

     
         
   
  • Tap Smart Tube on hard surface to ensure LyoSphere is in lower chamber and not clinging to inside of Cap

 
         
   
  • Write down how much time has elapsed since blood was drawn from donor

  • Blood should be used within 30 minutes of blood draw, if possible

 
         
   
  • It is important to minimize the time required to add blood to the set of three Smart Tubes

  • Remove the caps from the Smart Tubes

  • Line up the Smart Tubes in a rack

 
         
   
  • Rock Vacutainer at least 5 times

  • Remove stopper from Vacutainer

  • Be sure to comply with your institution's regulations for safely handling blood samples

 
         
   
  • Withdraw 1.0 milliliters of whole blood from Vacutainer

  • Place the pipette tip into the hole in the Smart Tube revealed by removing the Plug and dispense the blood into the Lower Chamber of the Smart Tube

  • IMPORTANT: ADD EXACTLY 1 MILLILITER OF BLOOD TO EACH SMART TUBE. ADDING MORE OR LESS THAN 1 MILLILITER OF BLOOD WILL CHANGE REACTION CHEMISTRY AND WILL MAKE SAMPLE UNRECOVERABLE

 
         
   
  • Image on the left shows blood in the Lower Chamber of Smart Tube

 
         
   
  • Screw Cap back onto Smart Tubes

 
         
 

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